@article {10471, title = {DEFB1 polymorphisms and salivary hBD-1 concentration in Oral Lichen Planus patients and healthy subjects.}, journal = {Arch Oral Biol}, volume = {73}, year = {2017}, month = {2017 Jan}, pages = {161-165}, abstract = {

OBJECTIVES: The aetiology of Oral Lichen Planus (OLP), a chronic inflammatory disease of oral mucosa, is not yet well understood. Since innate immunity may be hypothesized as involved in the susceptibility to OLP, we studied human beta defensin 1 (hBD-1) an antimicrobial peptide constitutively expressed in the saliva, looking at functional genetic variants possibly able to diminish hBD-1 production an consequently conferring major susceptibility to OLP.

DESIGN: We analysed three DEFB1 polymorphisms at 5{\textquoteright} UTR, -52G>A (rs1799946), -44C>G (rs1800972), -20G>A (rs11362) and two DEFB1 polymorphisms at 3{\textquoteright}UTR, c*5G>A (rs1047031), c*87A>G (rs1800971), with the aim of correlating these genetic variants and hBD-1 salivary level in a group of OLP patients and in healthy subjects. We also evaluated hBD-1 salivary concentrations, using ELISA, in OLP and healthy controls.

RESULTS: We compared hBD-1 concentrations in OLP and healthy subjects: hBD-1 concentration was significantly higher in OLP patients respect to control. When considering the correlation between DEFB1 polymorphisms genotypes and hBD-1 expression levels, significant results were obtained for SNPs -52G>A (p=0.03 both in OLP patients and healthy individuals) and -44C>G (p=0.02 in OLP patients).

CONCLUSIONS: hBD-1 production was different between OLP and healthy subjects (not age-matched with OLP). DEFB1 gene polymorphisms, -52G>A and -44C>G, correlated with hBD-1 salivary concentrations.

}, keywords = {5{\textquoteright} Untranslated Regions, Adult, Aged, Aged, 80 and over, beta-Defensins, Female, Genetic Predisposition to Disease, Genotype, Humans, Lichen Planus, Oral, Male, Middle Aged, Polymorphism, Single Nucleotide, Saliva, Sequence Analysis, DNA}, issn = {1879-1506}, doi = {10.1016/j.archoralbio.2016.10.008}, author = {Polesello, Vania and Zupin, Luisa and Di Lenarda, Roberto and Biasotto, Matteo and Pozzato, Gabriele and Ottaviani, Giulia and Gobbo, Margherita and Crovella, Sergio and Segat, Ludovica} }