@article {1972,
	title = {Comparison of hybrid capture II, linear array, and a bead-based multiplex genotyping assay for detection of human papillomavirus in women with negative pap test results and atypical squamous cells of undetermined significance.},
	journal = {J Clin Microbiol},
	volume = {50},
	year = {2012},
	month = {2012 Dec},
	pages = {4041-6},
	abstract = {<p>Many methods with different levels of analytical sensitivity and clinical specificity have been developed to detect the presence of high-risk (HR) types of the human papillomavirus (HPV) in cervical samples. The Hybrid Capture II (HC-II) assay is broadly used for primary screening. In addition, several HPV genotyping assays, based on PCR methods, display higher sensitivity than the HC-II and are also used in screening programs. We evaluated the performance of three HPV DNA tests, namely, the HC-II, the Linear Array (LA) HPV genotyping assay, and an HPV type-specific E7 PCR bead-based multiplex genotyping assay (TS-MPG) that is a laboratory-developed method for the detection of HPV, in 94 women with atypical squamous cells of undetermined significance (ASC-US) and in cytological samples from 86 women with a negative Pap test. The HPV prevalence with the TS-MPG assay was increased compared to the prevalence with the LA and HC-II assays. The HPV DNA prevalence in women with ASC-US was greater with the TS-MPG assay (46.2\%) than with the LA (36.3\%) and HC-II (29.7\%) assays. The HPV DNA prevalence in the control group was greater with the TS-MPG assay (32.1\%) than with the LA assay (10.7\%). Two women with ASC-US who were HPV DNA negative by the HC-II and positive by the TS-MPG or/and LA assays had lesions that progressed to low-grade squamous intraepithelial and high-grade squamous intraepithelial lesions. This study shows that the TS-MPG assay exhibited higher analytical sensitivity than the LA and HC-II assays for the detection of HPV DNA, which reduces the potential to incorrectly identify a woman{\textquoteright}s HPV infection status.</p>},
	keywords = {Adult, DNA, Viral, Female, Humans, Molecular Diagnostic Techniques, Papillomavirus Infections, Sensitivity and Specificity, Uterine Cervical Neoplasms, Virology},
	issn = {1098-660X},
	doi = {10.1128/JCM.02105-12},
	author = {Comar, Manola and Iannacone, Michelle R and Casalicchio, Giorgia and McKay-Chopin, Sandrine and Tommasino, Massimo and Gheit, Tarik}
}
