@article {8328,
	title = {In vitro sensitivity to methyl-prednisolone is associated with clinical response in pediatric idiopathic nephrotic syndrome.},
	journal = {Clin Pharmacol Ther},
	volume = {100},
	year = {2016},
	month = {2016 Sep},
	pages = {268-74},
	abstract = {<p>The aim of this study was to evaluate the in vitro steroid sensitivity as a predictor of clinical response to glucocorticoids in childhood idiopathic nephrotic syndrome (INS). Seventy-four patients (median age 4.33, interquartile range [IQR] 2.82-7.23; 63.5\% male) were enrolled in a prospective multicenter study: in vitro steroid inhibition of patients{\textquoteright} peripheral blood mononuclear cell proliferation was evaluated by [methyl-(3) H] thymidine incorporation assay at disease onset (T0) and after 4 weeks (T4) of treatment. Steroid dependence was associated with increased in vitro sensitivity at T4 assessed both as drug concentration inducing 50\% of inhibition (IC50 ; odds ratio [OR] = 0.48, 95\% confidence interval [CI] = 0.24-0.85; P = 0.0094) and maximum inhibition at the highest drug concentration (Imax ; OR = 1.13, 95\% CI = 1.02-1.31; P = 0.017). IC50 > 4.4 nM and Imax < 92\% at T4 were good predictors for optimal clinical response. These results suggest that this test may be useful for predicting the response to glucocorticoid therapy in pediatric INS.</p>},
	issn = {1532-6535},
	doi = {10.1002/cpt.372},
	author = {Cuzzoni, E and De Iudicibus, S and Stocco, G and Favretto, D and Pelin, M and Messina, G and Ghio, L and Monti, E and Pasini, A and Montini, G and Decorti, G}
}
