@article {10779,
	title = {Ferruginous bodies resolved by synchrotron XRF in a dog with peritoneal malignant mesothelioma.},
	journal = {Environ Sci Pollut Res Int},
	volume = {25},
	year = {2018},
	month = {2018 Dec},
	pages = {35707-35714},
	abstract = {<p>Mesothelioma is a malignant tumor mainly correlated to occupational asbestos exposure. Rare reports describe its occurrence also in animals, mainly linked to asbestos in the environment. Asbestos exposure is demonstrated by the appearance of characteristic histological hallmarks: asbestos containing ferruginous bodies that are iron-based structures forming around fibers and also other dust particles. Here we present a clinical case of a suspect of mesothelioma in the peritoneum of a dog with parallel histological observation of ferruginous bodies. To possibly correlate the dog tumor to environmental exposure, we performed X-ray fluorescence (XRF) analyses at two different synchrotrons to resolve the ferruginous bodies{\textquoteright} composition. While the histological examination diagnoses a tubulo-papillary mesothelioma, the XRF analyses show that ferruginous bodies contain Si particles, resembling formations of exogenous origin; however, the morphology is unlikely that of asbestos fibers. We speculate that the peritoneal mesothelioma of this dog could be related to environmental exposure to non-asbestos material.</p>},
	keywords = {Animals, Asbestos, Dogs, Environmental Exposure, Immunohistochemistry, Iron, Lung, Lung Neoplasms, Male, Mesothelioma, Peritoneal Neoplasms, Silicon, Spectrometry, X-Ray Emission, Synchrotrons},
	issn = {1614-7499},
	doi = {10.1007/s11356-018-3521-x},
	author = {Pascotto, Ernesto and Gianoncelli, Alessandra and Calligaro, Carla and Marcuzzo, Thomas and Melato, Mauro and Rizzardi, Clara and Pascolo, Lorella}
}
@article {7711,
	title = {Differential protein folding and chemical changes in lung tissues exposed to asbestos or particulates.},
	journal = {Sci Rep},
	volume = {5},
	year = {2015},
	month = {2015},
	pages = {12129},
	abstract = {<p>Environmental and occupational inhalants may induce a large number of pulmonary diseases, with asbestos exposure being the most risky. The mechanisms are clearly related to chemical composition and physical and surface properties of materials. A combination of X-ray fluorescence (μXRF) and Fourier Transform InfraRed (μFTIR) microscopy was used to chemically characterize and compare asbestos bodies versus environmental particulates (anthracosis) in lung tissues from asbestos exposed and control patients. μXRF analyses revealed heterogeneously aggregated particles in the anthracotic structures, containing mainly Si, K, Al and Fe. Both asbestos and particulates alter lung iron homeostasis, with a more marked effect in asbestos exposure. μFTIR analyses revealed abundant proteins on asbestos bodies but not on anthracotic particles. Most importantly, the analyses demonstrated that the asbestos coating proteins contain high levels of β-sheet structures. The occurrence of conformational changes in the proteic component of the asbestos coating provides new insights into long-term asbestos effects.</p>},
	issn = {2045-2322},
	doi = {10.1038/srep12129},
	author = {Pascolo, Lorella and Borelli, Violetta and Canzonieri, Vincenzo and Gianoncelli, Alessandra and Birarda, Giovanni and Bedolla, Diana E and Salom{\`e}, Murielle and Vaccari, Lisa and Calligaro, Carla and Cotte, Marine and Hesse, Bernhard and Luisi, Fernando and Zabucchi, Giuliano and Melato, Mauro and Rizzardi, Clara}
}
