@article {10439, title = {Vaginal microbiota dysmicrobism and role of biofilm-forming bacteria.}, journal = {Front Biosci (Elite Ed)}, volume = {10}, year = {2018}, month = {2018 06 01}, pages = {528-536}, abstract = {

Bacterial vaginosis involves the presence of a polymicrobial biofilm on the vaginal epithelium, guaranteeing immune escape and spread of antibiotic resistance. To spot known biofilm-forming bacteria, we profiled the vaginal microbiome of sixty-four symptomatic women suffering from a different grade of vaginal disorders and sixty asymptomatic healthy women. Specific microbial profiles distinguished symptomatic from asymptomatic women and characterized the grade of dysmicrobism within the symptomatic group. Lactobacillus crispatus and iners predominated on the healthy vaginal mucosa, while Lactobacillus gasseri predominated in the intermediate dysmicrobism. Furthermore, the intermediate grade of dysmicrobism was characterized by other lactic acid-producers species than Lactobacilli, able to rescue the microbial imbalance, and Ureaplasma parvum-serovar 3. The vaginosis group exhibited the overgrowth of Prevotella bivia, which is known to enhance the biofilm formation by Gardnerella vaginalis, and the presence of Streptococcus anginosus, which is emerging as a new cooperating player of the vaginal biofilm. Identifying specific microorganisms promoting or preventing the biofilm formation could increase the accuracy for a better definition of the vaginal dysmicrobism concept and therapeutic intervention.

}, keywords = {Adult, Biofilms, Female, Humans, Microbiota, Vagina, Vaginosis, Bacterial}, issn = {1945-0508}, author = {Campisciano, Giuseppina and Zanotta, Nunzia and Petix, Vincenzo and Corich, Lucia and De Seta, Francesco and Comar, Manola} } @article {3521, title = {Intestinal-mucosa anti-transglutaminase antibody assays to test for genetic gluten intolerance.}, journal = {Cell Mol Immunol}, volume = {11}, year = {2014}, month = {2014 Nov}, pages = {617-20}, keywords = {Celiac Disease, Cell Surface Display Techniques, Child, Diet, Gluten-Free, Disease Progression, Early Diagnosis, Female, Follow-Up Studies, HLA-DQ Antigens, Humans, Immunoassay, Immunoglobulin A, Intestinal Mucosa, Male, Prospective Studies, Transglutaminases}, issn = {2042-0226}, doi = {10.1038/cmi.2014.32}, author = {Quaglia, Sara and De Leo, Luigina and Ziberna, Fabiana and Vatta, Serena and Villanacci, Vincenzo and Granzotto, Marilena and Petix, Vincenzo and Martelossi, Stefano and Di Leo, Grazia and Torelli, Lucio and Not, Tarcisio} } @article {1877, title = {A real-time polymerase chain reaction-based protocol for low/medium-throughput Y-chromosome microdeletions analysis.}, journal = {Genet Test Mol Biomarkers}, volume = {16}, year = {2012}, month = {2012 Dec}, pages = {1349-55}, abstract = {

PURPOSE: We describe a real-time polymerase chain reaction (PCR) protocol based on the fluorescent molecule SYBR Green chemistry, for a low- to medium-throughput analysis of Y-chromosome microdeletions, optimized according to the European guidelines and aimed at making the protocol faster, avoiding post-PCR processing, and simplifying the results interpretation.

METHODS: We screened 156 men from the Assisted Reproduction Unit, Department of Obstetrics and Gynecology, Institute for Maternal and Child Health IRCCS Burlo Garofolo (Trieste, Italy), 150 not presenting Y-chromosome microdeletion, and 6 with microdeletions in different azoospermic factor (AZF) regions. For each sample, the Zinc finger Y-chromosomal protein (ZFY), sex-determining region Y (SRY), sY84, sY86, sY127, sY134, sY254, and sY255 loci were analyzed by performing one reaction for each locus.

RESULTS: AZF microdeletions were successfully detected in six individuals, confirming the results obtained with commercial kits.

CONCLUSION: Our real-time PCR protocol proved to be a rapid, safe, and relatively cheap method that was suitable for a low- to medium-throughput diagnosis of Y-chromosome microdeletion, which allows an analysis of approximately 10 samples (with the addition of positive and negative controls) in a 96-well plate format, or approximately 46 samples in a 384-well plate for all markers simultaneously, in less than 2 h without the need of post-PCR manipulation.

}, keywords = {Azoospermia, Chromosome Deletion, Chromosomes, Human, Y, Female, Humans, Kruppel-Like Transcription Factors, Male, Real-Time Polymerase Chain Reaction, Sex Chromosome Aberrations, Sex Chromosome Disorders of Sex Development}, issn = {1945-0257}, doi = {10.1089/gtmb.2012.0220}, author = {Segat, Ludovica and Padovan, Lara and Doc, Darja and Petix, Vincenzo and Morgutti, Marcello and Crovella, Sergio and Ricci, Giuseppe} }