@article {3486, title = {Influence of HLA-G polymorphisms in human immunodeficiency virus infection and hepatitis C virus co-infection in Brazilian and Italian individuals.}, journal = {Infect Genet Evol}, volume = {21}, year = {2014}, month = {2014 Jan}, pages = {418-23}, abstract = {

OBJECTIVE: This study aimed to investigate the role of Human Leukocyte Antigen (HLA)-G in the susceptibility to HIV-1 infection through the analysis of the HLA-G 3{\textquoteright} untranslated region (UTR) polymorphisms 14 bp insertion/deletion (rs66554220) and +3142C>G (rs1063320).

DESIGN: We analyzed 582 HIV-1 infected patients and 626 uninfected individuals from Brazil and Italy in a case-control study.

METHODS: HLA-G polymorphisms were genotyped using PCR, PCR-RFLP assays or direct sequencing. All analyses were stratified by ethnicity. Genotypic, allelic and diplotypic frequencies were compared between HIV-1 infected subjects and controls using Chi-square or Fischer exact tests. Also, haplotypic frequencies were estimated using MLocus software.

RESULTS: African-derived HIV-infected individuals presented a higher frequency of the 14 bp insertion allele as compared to non-infected individuals (0.468 versus 0.373, respectively; p(Bonf) = 0.010). A higher frequency of the 14 bp insertion +3142G (insG) haplotype (0.456 versus 0.346, p<0.001) and the insG/insG diplotype (OR=1.88, 95\%CI = 1.08-3.23, p=0.021) was observed among African-derived patients as compared to uninfected controls. Also, we observed a higher frequency of the ins/ins genotype among African-derived HIV patients co-infected with HCV (OR=2.78, 95\%CI = 1.20-6.49, p = 0.008).

CONCLUSIONS: Our data point out to an increased frequency of alleles and genotypes associated with low HLA-G expression among African-derived patients, suggesting a potential role for HLA-G in the susceptibility to HIV-1 infection and HCV co-infection in those individuals.

}, keywords = {3{\textquoteright} Untranslated Regions, Adolescent, Adult, African Continental Ancestry Group, Aged, Brazil, Coinfection, Gene Frequency, Genetic Variation, Haplotypes, Hepatitis C, HIV Infections, HLA-G Antigens, Humans, Italy, Male, Middle Aged, Polymorphism, Single Nucleotide, Young Adult}, issn = {1567-7257}, doi = {10.1016/j.meegid.2013.12.013}, author = {da Silva, G K and Vianna, Priscila and Veit, Tiago Degani and Crovella, Sergio and Catamo, Eulalia and Cordero, Elvira Alicia Aparicio and Mattevi, Vanessa Su{\~n}{\'e} and Lazzaretti, Rosmeri Kuhmmer and Sprinz, Eduardo and Kuhmmer, Regina and Chies, Jos{\'e} Artur Bogo} }