@article {1634, title = {High polymorphism of the MBL2 gene in patients with atopic dermatitis.}, journal = {Ann Allergy Asthma Immunol}, volume = {105}, year = {2010}, month = {2010 Jul}, pages = {39-42}, abstract = {

BACKGROUND: Low serum levels of mannose-binding lectin (MBL) are determined mainly by variant alleles of the MBL2 gene and it has been suggested that MBL may play a role in the susceptibility to atopic dermatitis (AD).

OBJECTIVE: The aim was to investigate the difference of the frequency of MBL2 variant alleles in AD patients and in a group of individuals without AD, and associate the MBL2 alleles with AD severity.

METHODS: MBL2 variant allele{\textquoteright}s frequency was investigated in 131 children with AD and 165 healthy children/adolescents matched by convenience. The severity of disease was graded according to the SCORing Atopic Dermatitis (SCORAD) index. The first exon variants were called "O" and the wild type "A". The variants in the promoter were H/L at -550 and X/Y at -221, determined by Real Time PCR.

RESULTS: Children with AD had higher frequency of allele O and the genotypes related to low or deficient levels of MBL, when compared to the healthy group (p = 0.0012 and p < 0.001, respectively), but not with AD severity.

CONCLUSION: Low or deficient MBL serum levels determined genetically may contribute to the predisposition for AD, but not for disease severity.

}, keywords = {Adolescent, Alleles, Child, Child, Preschool, Dermatitis, Atopic, Disease Progression, Female, Genetic Predisposition to Disease, Genotype, Humans, Infant, Male, Mannose-Binding Lectin, Polymorphism, Genetic, Promoter Regions, Genetic}, issn = {1081-1206}, doi = {10.1016/j.anai.2010.03.017}, author = {Carr{\'e}ra, Matilde Campos and Moura, Patr{\'\i}cia and Crovella, Sergio and de Souza, Paulo Roberto Eleut{\'e}rio and de Alencar, Luiz Cl{\'a}udio Arraes and Sarinho, Emanuel} } @article {1759, title = {[Human papillomavirus cervical infection: viral genotyping and risk factors for high-grade squamous intraepithelial lesion and cervix cancer].}, journal = {Rev Bras Ginecol Obstet}, volume = {32}, year = {2010}, month = {2010 Oct}, pages = {476-85}, abstract = {

PURPOSE: to analyze the characteristics of viral infection and the risk factors for high-grade squamous intraepithelial lesion and cervical carcinoma in women with cervical HPV infection.

METHODS: a case-control study was conducted on women with cervical HPV at a Gynecology reference service enrolled at the Public Health System, located in Recife, Northeastern Brazil. The groups of cases (72 women with high-grade squamous intraepithelial lesion or cervical cancer) and controls (176 women with normal Pap smear or benign alterations) were investigated for six viral genotypes (HPV 16, 18, 31, 33, 6, 11) in ecto- and endocervical material using MY09/MY11 primers. The independent variables were ranked in three levels of determination: distal (sociodemographic), intermediate (behavioral) and proximal (previous Pap smear). The homogeneity of proportions was tested (χ2), unadjusted Odds Ratios (OR) were obtained and hierarchical logistic regression was applied to the final model, with adjustment of the effect of each variable to the outcome based on the variables in the same and previous levels of causality.

RESULTS: the viral genotype of cervical infection was identified in 76.6\% of the 248 women participating in the study. High-risk HPV genotypes (83.4\% of cases and 67.1\% of controls) were predominant, especially HPV 16 and 31. The distal risk factors identified were: living in a rural area (OR=2.71, 95\%CI: 1.18-6.23), less than three years of study (OR=3.97, 95\%CI: 2.09-7.54) and family income below two minimum wages (OR=3.30, 95\%CI: 1.04-10.51); intermediate: four or more pregnancies (OR=2.00, 95\%CI: 1.06-3.76); and proximal: absence of a previous Pap smear (OR=9.74, 95\%CI: 2.48-38.28).

CONCLUSIONS: genotypes 16 and 31 of cervical HPV infection are predominant among women assisted by the Public Health System in Northeastern Brazil. Socioeconomic and reproductive factors, as well as the absence of cytological screening, represent risk factors for the progression of infection to high-grade squamous intraepithelial lesion and cervical cancer.

}, keywords = {Adult, Carcinoma in Situ, Case-Control Studies, Female, Genotype, Humans, Papillomaviridae, Papillomavirus Infections, Risk Factors, Socioeconomic Factors, Uterine Cervical Neoplasms}, issn = {1806-9339}, author = {de Mendon{\c c}a, Vilma Guimar{\~a}es and Guimar{\~a}es, Maria Jos{\'e} Bezerra and de Lima Filho, Jos{\'e} Luiz and de Mendon{\c c}a, Carolina Guimar{\~a}es and Martins, Danyelly Bruneska Gondim and Crovella, Sergio and de Alencar, Luiz Cl{\'a}udio Arraes} }