@article {10803,
	title = {Interstitial Fluid in Gynecologic Tumors and Its Possible Application in the Clinical Practice.},
	journal = {Int J Mol Sci},
	volume = {19},
	year = {2018},
	month = {2018 Dec 12},
	abstract = {<p>Gynecologic cancers are an important cause of worldwide mortality. The interstitium consists of solid and fluid phases, situated between the blood vessels and cells. The interstitial fluid (IF), or fluid phase, is an extracellular fluid bathing and surrounding the tissue cells. The TIF (tumor interstitial fluid) is a dynamic fluid rich in lipids, proteins and enzyme-derived substances. The molecules found in the IF may be associated with pathological changes in tissues leading to cancer growth and metastatization. Proteomic techniques have allowed an extensive study of the composition of the TIF as a source of biomarkers for gynecologic cancers. In our review, we analyze the composition of the TIF, its formation process, the sampling methods, the consequences of its accumulation and the proteomic analyses performed, that make TIF valuable for monitoring different types of cancers.</p>},
	keywords = {Biomarkers, Tumor, Biophysical Phenomena, Extracellular Fluid, Female, Genital Neoplasms, Female, Humans, Practice Patterns, Physicians{\textquoteright}, Tumor Microenvironment},
	issn = {1422-0067},
	doi = {10.3390/ijms19124018},
	author = {Ura, Blendi and Di Lorenzo, Giovanni and Romano, Federico and Monasta, Lorenzo and Mirenda, Giuseppe and Scrimin, Federica and Ricci, Giuseppe}
}
@article {10542,
	title = {Identification of proteins with different abundance associated with cell migration and proliferation in leiomyoma interstitial fluid by proteomics.},
	journal = {Oncol Lett},
	volume = {13},
	year = {2017},
	month = {2017 May},
	pages = {3912-3920},
	abstract = {<p>Uterine leiomyoma is the most common female reproductive tract benign tumor. Little is known about protein composition and changes in the leiomyoma interstitial fluid (IF). The present study focused on changes in protein abundance in the IF of leiomyoma. Leiomyoma IFs and adjacent myometrial IFs were obtained and analyzed by two-dimensional electrophoresis (2-DE) coupled with mass spectrometry and western blotting for 2-DE data validation. A total of 25 unique proteins were observed to change significantly (P<0.05). Of these proteins with different abundance, 22 had not been previously identified in leiomyoma IF.  analysis predicted that three of these proteins were secreted via classical mechanisms, while 22 were secreted via non-classical mechanisms. Ingenuity Pathway Analysis identified 17 proteins associated with cellular migration and proliferation. Among these, phosphoglycerate mutase 1 had not been previously associated with leiomyoma. The abundance of seven proteins was further validated by western blotting. A comparative proteomic approach identified a number of proteins associated with cellular migration and proliferation, with changes in abundance in IF likely to be involved in tumor development. Further studies will be required to investigate the role of these proteins in leiomyoma IF and their possible association with tumor development and growth.</p>},
	issn = {1792-1074},
	doi = {10.3892/ol.2017.5943},
	author = {Ura, Blendi and Scrimin, Federica and Franchin, Cinzia and Arrigoni, Giorgio and Licastro, Danilo and Monasta, Lorenzo and Ricci, Giuseppe}
}
