@article {8493, title = {Anti-ApoA-1 IgG serum levels predict worse poststroke outcomes.}, journal = {Eur J Clin Invest}, volume = {46}, year = {2016}, month = {2016 Sep}, pages = {805-17}, abstract = {

BACKGROUND: Autoantibodies to apolipoprotein A-1 (anti-ApoA-1 IgG) were shown to predict major adverse cardiovascular events and promote atherogenesis. However, their potential relationship with clinical disability and ischaemic lesion volume after acute ischaemic stroke (AIS) remains unexplored.

MATERIALS AND METHODS: We included n = 76 patients admitted for AIS and we investigated whether baseline serum anti-ApoA-1 IgG levels could predict (i) AIS-induced clinical disability [assessed by the modified Rankin Scale (mRS)], and (ii) AIS-related ischaemic lesion volume [assessed by Computed Tomography (CT)]. We also evaluated the possible pro-apoptotic and pro-necrotic effects of anti-ApoA-1 IgG on human astrocytoma cell line (U251) using flow cytometry.

RESULTS: High levels of anti-ApoA-1 IgG were retrieved in 15{\textperiodcentered}8\% (12/76) of patients. Increased baseline levels of anti-ApoA-1 IgG were independently correlated with worse mRS [β = 0{\textperiodcentered}364; P = 0{\textperiodcentered}002; adjusted odds ratio (OR): 1{\textperiodcentered}05 (95\% CI 1{\textperiodcentered}01-1{\textperiodcentered}09); P = 0{\textperiodcentered}017] and CT-assessed ischaemic lesion volume [β = 0{\textperiodcentered}333; P < 0{\textperiodcentered}001; adjusted OR: 1{\textperiodcentered}06 (95\% CI 1{\textperiodcentered}01-1{\textperiodcentered}12); P = 0{\textperiodcentered}048] at 3 months. No difference in baseline clinical, biochemical and radiological characteristics was observed between patients with high vs. low levels of anti-ApoA-1 IgG. Incubating human astrocytoma cells with anti-ApoA-1 IgG dose dependently induced necrosis and apoptosis of U251 cells in vitro.

CONCLUSION: Anti-ApoA-1 IgG serum levels at AIS onset are associated with poorer clinical recovery and worse brain lesion volume 3 months after AIS. These observations could be partly explained by the deleterious effect of anti-ApoA-1 IgG on human brain cell survival in vitro and may have clinical implication in the prediction of poor outcome in AIS.

}, issn = {1365-2362}, doi = {10.1111/eci.12664}, author = {Carbone, Federico and Satta, Nathalie and Montecucco, Fabrizio and Virzi, Julien and Burger, Fabienne and Roth, Aline and Roversi, Gloria and Tamborino, Carmine and Casetta, Ilaria and Seraceni, Silva and Trentini, Alessandro and Padroni, Marina and Dallegri, Franco and Lalive, Patrice H and Mach, Fran{\c c}ois and Fainardi, Enrico and Vuilleumier, Nicolas} } @article {7713, title = {Association Study between Cervical Lesions and Single or Multiple Vaccine-Target and Non-Vaccine Target Human Papillomavirus (HPV) Types in Women from Northeastern Brazil.}, journal = {PLoS One}, volume = {10}, year = {2015}, month = {2015}, pages = {e0132570}, abstract = {

We performed an association between high-grade squamous intraepithelial lesions (HSIL), low-grade squamous intraepithelial lesions (LSIL) and single or multiple vaccine-target as well as non-vaccine target Human papillomavirus (HPV) types. Using bead-based HPV genotyping, 594 gynecological samples were genotyped. An association between squamous intraepithelial lesion (SIL) and presence of HPV16, 18, 31, 58 and 56 types were calculated. The risk was estimated by using odds ratio (OR) and 95\% of confidence intervals (CI). A total of 370 (62.3\%) women were HPV positive. Among these, 157 (42.7\%) presented a single HPV infection, and 212 (57.3\%) were infected by more than one HPV type. HPV31 was the most prevalent genotype, regardless single and multiple HPV infections. Single infection with HPV31 was associated with LSIL (OR=2.32; 95\%CI: 1.01 to 5.32; p=0.04); HPV31 was also associated with LSIL (OR=3.28; 95\%CI: 1.74 to 6.19; p= 0.0002) and HSIL (OR=3.82; 95\%CI: 2.10 to 6.97; p<0.001) in multiple HPV infections. Risk to harbor cervical lesions was observed in multiple HPV infections with regard to the HPV56 (OR=5.39; 95\%CI: 2.44 to 11.90; p<0.001for LSIL; OR=5.37; 95\%CI: 2.71 to 10.69; p<0.001) and HPV58 (OR=3.29; 95\%CI: 1.34 to 8.09; p=0.0091 for LSIL; OR=3.55; 95\%CI: 1.56 to 8.11; p=0.0026) genotypes. In addition, women coinfected with HPV16/31/56 types had 6 and 5-fold increased risk of HSIL (OR=6.46; 95\%CI: 1.89 to 22.09; p=0.002) and LSIL (OR=5.22; 95\%CI: 1.10 to 24.70; p=0.03), respectively. Multiple HPV infections without HPV16/18 has 2-fold increased risk of HSIL (OR=2.57; 95\%CI: 1.41 to 4.70; p=0.002) and LSIL OR=2.03; 95\%CI: 1.08 to 3.79; p=0.02). The results of this study suggest that single and multiple vaccine target as well as non-vaccine target HPV types are associated with LSIL and HSIL. These finding should be taken into consideration in the design of HPV vaccination strategies.

}, issn = {1932-6203}, doi = {10.1371/journal.pone.0132570}, author = {Chagas, B{\'a}rbara Simas and Comar, Manola and Gurgel, Ana Pavla Almeida Diniz and Paiva, S{\'e}rgio and Seraceni, Silva and de Freitas, Antonio Carlos and Crovella, Sergio} }