TY - JOUR T1 - DEFB1 polymorphisms and salivary hBD-1 concentration in Oral Lichen Planus patients and healthy subjects. JF - Arch Oral Biol Y1 - 2017 A1 - Polesello, Vania A1 - Zupin, Luisa A1 - Di Lenarda, Roberto A1 - Biasotto, Matteo A1 - Pozzato, Gabriele A1 - Ottaviani, Giulia A1 - Gobbo, Margherita A1 - Crovella, Sergio A1 - Segat, Ludovica KW - 5' Untranslated Regions KW - Adult KW - Aged KW - Aged, 80 and over KW - beta-Defensins KW - Female KW - Genetic Predisposition to Disease KW - Genotype KW - Humans KW - Lichen Planus, Oral KW - Male KW - Middle Aged KW - Polymorphism, Single Nucleotide KW - Saliva KW - Sequence Analysis, DNA AB -

OBJECTIVES: The aetiology of Oral Lichen Planus (OLP), a chronic inflammatory disease of oral mucosa, is not yet well understood. Since innate immunity may be hypothesized as involved in the susceptibility to OLP, we studied human beta defensin 1 (hBD-1) an antimicrobial peptide constitutively expressed in the saliva, looking at functional genetic variants possibly able to diminish hBD-1 production an consequently conferring major susceptibility to OLP.

DESIGN: We analysed three DEFB1 polymorphisms at 5' UTR, -52G>A (rs1799946), -44C>G (rs1800972), -20G>A (rs11362) and two DEFB1 polymorphisms at 3'UTR, c*5G>A (rs1047031), c*87A>G (rs1800971), with the aim of correlating these genetic variants and hBD-1 salivary level in a group of OLP patients and in healthy subjects. We also evaluated hBD-1 salivary concentrations, using ELISA, in OLP and healthy controls.

RESULTS: We compared hBD-1 concentrations in OLP and healthy subjects: hBD-1 concentration was significantly higher in OLP patients respect to control. When considering the correlation between DEFB1 polymorphisms genotypes and hBD-1 expression levels, significant results were obtained for SNPs -52G>A (p=0.03 both in OLP patients and healthy individuals) and -44C>G (p=0.02 in OLP patients).

CONCLUSIONS: hBD-1 production was different between OLP and healthy subjects (not age-matched with OLP). DEFB1 gene polymorphisms, -52G>A and -44C>G, correlated with hBD-1 salivary concentrations.

VL - 73 U1 - http://www.ncbi.nlm.nih.gov/pubmed/27770642?dopt=Abstract ER -