TY - JOUR T1 - Genome-wide association analysis on normal hearing function identifies PCDH20 and SLC28A3 as candidates for hearing function and loss. JF - Hum Mol Genet Y1 - 2015 A1 - Vuckovic, Dragana A1 - Dawson, Sally A1 - Scheffer, Deborah I A1 - Rantanen, Taina A1 - Morgan, Anna A1 - Di Stazio, Mariateresa A1 - Vozzi, Diego A1 - Nutile, Teresa A1 - Concas, Maria P A1 - Biino, Ginevra A1 - Nolan, Lisa A1 - Bahl, Aileen A1 - Loukola, Anu A1 - Viljanen, Anne A1 - Davis, Adrian A1 - Ciullo, Marina A1 - Corey, David P A1 - Pirastu, Mario A1 - Gasparini, Paolo A1 - Girotto, Giorgia AB -

Hearing loss and individual differences in normal hearing both have a substantial genetic basis. Although many new genes contributing to deafness have been identified, very little is known about genes/variants modulating the normal range of hearing ability. To fill this gap, we performed a two-stage meta-analysis on hearing thresholds (tested at 0.25, 0.5, 1, 2, 4, 8 kHz) and on pure-tone averages (low-, medium- and high-frequency thresholds grouped) in several isolated populations from Italy and Central Asia (total N = 2636). Here, we detected two genome-wide significant loci close to PCDH20 and SLC28A3 (top hits: rs78043697, P = 4.71E-10 and rs7032430, P = 2.39E-09, respectively). For both loci, we sought replication in two independent cohorts: B58C from the UK (N = 5892) and FITSA from Finland (N = 270). Both loci were successfully replicated at a nominal level of significance (P < 0.05). In order to confirm our quantitative findings, we carried out RT-PCR and reported RNA-Seq data, which showed that both genes are expressed in mouse inner ear, especially in hair cells, further suggesting them as good candidates for modulatory genes in the auditory system. Sequencing data revealed no functional variants in the coding region of PCDH20 or SLC28A3, suggesting that variation in regulatory sequences may affect expression. Overall, these results contribute to a better understanding of the complex mechanisms underlying human hearing function.

VL - 24 IS - 19 U1 - http://www.ncbi.nlm.nih.gov/pubmed/26188009?dopt=Abstract ER -