TY - JOUR T1 - Frequency of HLA B*5701 allele carriers in abacavir treated-HIV infected patients and controls from northeastern Brazil. JF - Clinics (Sao Paulo) Y1 - 2011 A1 - Crovella, Sergio A1 - Biller, Lara A1 - Santos, Sergio A1 - Salustiano, Ana A1 - Brandão, Lucas A1 - Guimarães, Rafael A1 - Segat, Ludovica A1 - Lima Filho, Jose Luiz de A1 - Arraes, Luiz Claudio KW - Adolescent KW - Adult KW - Anti-HIV Agents KW - Brazil KW - Case-Control Studies KW - Dideoxynucleosides KW - Drug Hypersensitivity KW - Female KW - Gene Frequency KW - Genotype KW - HIV Infections KW - HLA-B Antigens KW - Humans KW - Male KW - Middle Aged KW - Real-Time Polymerase Chain Reaction KW - Young Adult VL - 66 IS - 8 U1 - http://www.ncbi.nlm.nih.gov/pubmed/21915505?dopt=Abstract ER - TY - JOUR T1 - The role of mannose-binding lectin gene polymorphisms in susceptibility to HIV-1 infection in Southern Brazilian patients. JF - AIDS Y1 - 2011 A1 - da Silva, Gabriela Kniphoff A1 - Guimarães, Rafael A1 - Mattevi, Vanessa Suñé A1 - Lazzaretti, Rosmeri Kuhmmer A1 - Sprinz, Eduardo A1 - Kuhmmer, Regina A1 - Brandão, Lucas A1 - Crovella, Sergio A1 - Chies, José Artur Bogo KW - Adult KW - Aged KW - Brazil KW - Female KW - Genetic Predisposition to Disease KW - HIV Infections KW - HIV-1 KW - Humans KW - Lectins, C-Type KW - Male KW - Mannose-Binding Lectin KW - Mannose-Binding Lectins KW - Middle Aged KW - Polymorphism, Genetic KW - Promoter Regions, Genetic KW - Receptors, Cell Surface KW - Young Adult AB -

OBJECTIVE: This study investigates the role of mannose-binding lectin (MBL) in the susceptibility to HIV-1 infection analyzing polymorphisms located at the MBL2 promoter and exon 1 regions.

MATERIALS AND METHODS: The prevalence of MBL2 variant alleles was investigated in 410 HIV-1-infected patients from the South Brazilian HIV cohort and in 345 unexposed uninfected healthy individuals. The promoter variants were genotyped using polymerase chain reaction with sequence-specific primers (PCR-SSP) and exon 1 variants were analyzed by real-time PCR using a melting temperature assay and were confirmed by PCR-restriction fragment length polymorphism (RFLP). MBL2 genotypic and allelic frequencies were compared between HIV-1-infected patients and controls using the chi-squared tests.

RESULTS: The analyses were performed subdividing the individuals according to their ethnic origin. Among Euro-derived individuals a higher frequency of the LX/LX genotype was observed in patients when compared to controls (P < 0.001). The haplotypic analysis also showed a higher frequency of the haplotypes associated with lower MBL levels among HIV-1-infected patients (P = 0.0001). Among Afro-derived individuals the frequencies of LY/LY and HY/HY genotypes were higher in patients when compared to controls (P = 0.009 and P = 0.02).

CONCLUSIONS: An increased frequency of MBL2 genotypes associated with low MBL levels was observed in Euro-derived patients, suggesting a potential role for MBL in the susceptibility to HIV-1 infection in Euro-derived individuals.

VL - 25 IS - 4 U1 - http://www.ncbi.nlm.nih.gov/pubmed/21192229?dopt=Abstract ER -