TY - JOUR T1 - Iron-related toxicity of single-walled carbon nanotubes and crocidolite fibres in human mesothelial cells investigated by Synchrotron XRF microscopy. JF - Sci Rep Y1 - 2018 A1 - Cammisuli, Francesca A1 - Giordani, Silvia A1 - Gianoncelli, Alessandra A1 - Rizzardi, Clara A1 - Radillo, Lucia A1 - Zweyer, Marina A1 - Da Ros, Tatiana A1 - Salomè, Murielle A1 - Melato, Mauro A1 - Pascolo, Lorella KW - Asbestos, Crocidolite KW - Cell Line KW - Epithelial Cells KW - Humans KW - Iron KW - Microscopy, Fluorescence KW - Nanotubes, Carbon AB -

Carbon nanotubes (CNTs) are promising products in industry and medicine, but there are several human health concerns since their fibrous structure resembles asbestos. The presence of transition metals, mainly iron, in the fibres seems also implicated in the pathogenetic mechanisms. To unravel the role of iron at mesothelial level, we compared the chemical changes induced in MeT-5A cells by the exposure to asbestos (crocidolite) or CNTs at different content of iron impurities (raw-SWCNTs, purified- and highly purified-SWCNTs). We applied synchrotron-based X-Ray Fluorescence (XRF) microscopy and soft X-ray imaging (absorption and phase contrast images) to monitor chemical and morphological changes of the exposed cells. In parallel, we performed a ferritin assay. X-ray microscopy imaging and XRF well localize the crocidolite fibres interacting with cells, as well as the damage-related morphological changes. Differently, CNTs presence could be only partially evinced by low energy XRF through carbon distribution and sometimes iron co-localisation. Compared to controls, the cells treated with raw-SWCNTs and crocidolite fibres showed a severe alteration of iron distribution and content, with concomitant stimulation of ferritin production. Interestingly, highly purified nanotubes did not altered iron metabolism. The data provide new insights for possible CNTs effects at mesothelial/pleural level in humans.

VL - 8 IS - 1 U1 - http://www.ncbi.nlm.nih.gov/pubmed/29335462?dopt=Abstract ER - TY - JOUR T1 - The Great Pretender: Pediatric Wandering Spleen: Two Case Reports and Review of the Literature. JF - Pediatr Emerg Care Y1 - 2016 A1 - Radillo, Lucia A1 - Taddio, Andrea A1 - Ghirardo, Sergio A1 - Bramuzzo, Matteo A1 - Pederiva, Federica A1 - Maschio, Massimo A1 - Barbi, Egidio AB -

Wandering spleen is a rare condition, typically not only due to embryological defects of the splenic ligaments, but also secondary to trauma and splenomegaly. The most common presentation is acute abdomen with a mobile abdominal mass or recurrent abdominal pain. However, the spleen may be temporary in its normal position, and patients could be asymptomatic. A familiarity, if present, strengthens the diagnostic suspect.Abdominal ultrasonography and computed tomography are the examination of choice, and the management is surgical.

U1 - http://www.ncbi.nlm.nih.gov/pubmed/27248774?dopt=Abstract ER -