TY - JOUR T1 - Cubosomes for in vivo fluorescence lifetime imaging. JF - Nanotechnology Y1 - 2017 A1 - Biffi, Stefania A1 - Andolfi, Laura A1 - Caltagirone, Claudia A1 - Garrovo, Chiara A1 - Falchi, Angela M A1 - Lippolis, Vito A1 - Lorenzon, Andrea A1 - Macor, Paolo A1 - Meli, Valeria A1 - Monduzzi, Maura A1 - Obiols-Rabasa, Marc A1 - Petrizza, Luca A1 - Prodi, Luca A1 - Rosa, Antonella A1 - Schmidt, Judith A1 - Talmon, Yeshayahu A1 - Murgia, Sergio KW - Animals KW - Carbocyanines KW - Cell Survival KW - Drug Compounding KW - Erythrocytes KW - Female KW - Fluorescent Dyes KW - Glycerides KW - Humans KW - Injections, Intravenous KW - Liposomes KW - Liver KW - Mice KW - Mice, Inbred BALB C KW - Nanoparticles KW - NIH 3T3 Cells KW - Optical Imaging KW - Particle Size KW - Spectroscopy, Near-Infrared KW - Time-Lapse Imaging AB -

Herein we provided the first proof of principle for in vivo fluorescence optical imaging application using monoolein-based cubosomes in a healthy mouse animal model. This formulation, administered at a non-cytotoxic concentration, was capable of providing both exogenous contrast for NIR fluorescence imaging with very high efficiency and chemospecific information upon lifetime analysis. Time-resolved measurements of fluorescence after the intravenous injection of cubosomes revealed that the dye rapidly accumulated mainly in the liver, while lifetimes profiles obtained in vivo allowed for discriminating between free dye or dye embedded within the cubosome nanostructure after injection.

VL - 28 IS - 5 U1 - http://www.ncbi.nlm.nih.gov/pubmed/28032617?dopt=Abstract ER -