TY - JOUR T1 - Cell-autonomous and cell non-autonomous downregulation of tumor suppressor DAB2IP by microRNA-149-3p promotes aggressiveness of cancer cells. JF - Cell Death Differ Y1 - 2018 A1 - Bellazzo, Arianna A1 - Di Minin, Giulio A1 - Valentino, Elena A1 - Sicari, Daria A1 - Torre, Denis A1 - Marchionni, Luigi A1 - Serpi, Federica A1 - Stadler, Michael B A1 - Taverna, Daniela A1 - Zuccolotto, Gaia A1 - Montagner, Isabella Monia A1 - Rosato, Antonio A1 - Tonon, Federica A1 - Zennaro, Cristina A1 - Agostinis, Chiara A1 - Bulla, Roberta A1 - Mano, Miguel A1 - Del Sal, Giannino A1 - Collavin, Licio AB -

The tumor suppressor DAB2IP contributes to modulate the network of information established between cancer cells and tumor microenvironment. Epigenetic and post-transcriptional inactivation of this protein is commonly observed in multiple human malignancies, and can potentially favor progression of tumors driven by a variety of genetic mutations. Performing a high-throughput screening of a large collection of human microRNA mimics, we identified miR-149-3p as a negative post-transcriptional modulator of DAB2IP. By efficiently downregulating DAB2IP, this miRNA enhances cancer cell motility and invasiveness, facilitating activation of NF-kB signaling and promoting expression of pro-inflammatory and pro-angiogenic factors. In addition, we found that miR-149-3p secreted by prostate cancer cells induces DAB2IP downregulation in recipient vascular endothelial cells, stimulating their proliferation and motility, thus potentially remodeling the tumor microenvironment. Finally, we found that inhibition of endogenous miR-149-3p restores DAB2IP activity and efficiently reduces tumor growth and dissemination of malignant cells. These observations suggest that miR-149-3p can promote cancer progression via coordinated inhibition of DAB2IP in tumor cells and in stromal cells.

VL - 25 IS - 7 U1 - http://www.ncbi.nlm.nih.gov/pubmed/29568059?dopt=Abstract ER -