%0 Journal Article %J Int J Nanomedicine %D 2015 %T Targeted tumor imaging of anti-CD20-polymeric nanoparticles developed for the diagnosis of B-cell malignancies. %A Capolla, Sara %A Garrovo, Chiara %A Zorzet, Sonia %A Lorenzon, Andrea %A Rampazzo, Enrico %A Spretz, Ruben %A Pozzato, Gabriele %A Núñez, Luis %A Tripodo, Claudio %A Macor, Paolo %A Biffi, Stefania %X

The expectations of nanoparticle (NP)-based targeted drug delivery systems in cancer, when compared with convectional therapeutic methods, are greater efficacy and reduced drug side effects due to specific cellular-level interactions. However, there are conflicting literature reports on enhanced tumor accumulation of targeted NPs, which is essential for translating their applications as improved drug-delivery systems and contrast agents in cancer imaging. In this study, we characterized biodegradable NPs conjugated with an anti-CD20 antibody for in vivo imaging and drug delivery onto tumor cells. NPs' binding specificity mediated by anti-CD20 antibody was evaluated on MEC1 cells and chronic lymphocytic leukemia patients' cells. The whole-body distribution of untargeted NPs and anti-CD20 NPs were compared by time-domain optical imaging in a localized human/mouse model of B-cell malignancy. These studies provided evidence that NPs' functionalization by an anti-CD20 antibody improves tumor pharmacokinetic profiles in vivo after systemic administration and increases in vivo imaging of tumor mass compared to non-targeted NPs. Together, drug delivery and imaging probe represents a promising theranostics tool for targeting B-cell malignancies.

%B Int J Nanomedicine %V 10 %P 4099-109 %8 2015 %G eng %1 http://www.ncbi.nlm.nih.gov/pubmed/26124662?dopt=Abstract %R 10.2147/IJN.S78995