%0 Journal Article %J J Neurovirol %D 2012 %T Specific protein profile in cerebrospinal fluid from HIV-1-positive cART-treated patients affected by neurological disorders. %A Zanin, Valentina %A Delbue, Serena %A Marcuzzi, Annalisa %A Tavazzi, Eleonora %A Del Savio, Rossella %A Crovella, Sergio %A Marchioni, Enrico %A Ferrante, Pasquale %A Comar, Manola %K Adult %K Aged %K Anti-HIV Agents %K Cytokines %K Encephalomyelitis, Acute Disseminated %K Female %K HIV Infections %K HIV-1 %K Humans %K Leukoencephalopathy, Progressive Multifocal %K Male %K Middle Aged %K Pilot Projects %X

Cytokines/chemokines are involved in the immune response of infections, including HIV-1. We defined the profile of 48 cytokines/chemokines in cerebrospinal fluid from 18 cART patients with chronic HIV-1 infection by Luminex technology. Nine patients were affected with leukoencephalopathies: five with John Cunningham virus (JCV) + progressive multifocal leukoencephalopathy (PML) and four with JCV-not determined leukoencephalopathy (NDLE). In addition, nine HIV-1-positive patients with no neurological signs (NND) and five HIV-1-negative patients affected with acute disseminated encephalomyelitis (ADEM) were enrolled. Ten cytokines (IL-15, IL-3, IL-16, IL-18, CTACK, GRO1, SCF, MCP-1, MIF, SDF) were highly expressed in HIV-1-positive patients while IL-1Ra and IL-17 were present at a lower level. In addition, the levels of IL-17, IL-9, FGF-basic, MIP-1β, and MCP-1 were significantly higher (p < 0.05) in patients with neurological diseases (PML, NDLE, ADEM) with respect to NND. Focusing the attention to the cytokine profile in JCV + PML patients with respect to JCV-NDLE patients, only TNF-β was significantly downregulated (p < 0.05) in JCV + PML patients. This pilot study emphasized the role of immunoregulation in HIV-1-related neurological disorders during cART treatment.

%B J Neurovirol %V 18 %P 416-22 %8 2012 Oct %G eng %N 5 %1 http://www.ncbi.nlm.nih.gov/pubmed/22581428?dopt=Abstract %R 10.1007/s13365-012-0109-y