<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Boding, Lasse</style></author><author><style face="normal" font="default" size="100%">Hansen, Ann K</style></author><author><style face="normal" font="default" size="100%">Meroni, Germana</style></author><author><style face="normal" font="default" size="100%">Johansen, Bo B</style></author><author><style face="normal" font="default" size="100%">Braunstein, Thomas H</style></author><author><style face="normal" font="default" size="100%">Bonefeld, Charlotte M</style></author><author><style face="normal" font="default" size="100%">Kongsbak, Martin</style></author><author><style face="normal" font="default" size="100%">Jensen, Benjamin A H</style></author><author><style face="normal" font="default" size="100%">Woetmann, Anders</style></author><author><style face="normal" font="default" size="100%">Thomsen, Allan R</style></author><author><style face="normal" font="default" size="100%">Odum, Niels</style></author><author><style face="normal" font="default" size="100%">von Essen, Marina R</style></author><author><style face="normal" font="default" size="100%">Geisler, Carsten</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Midline 1 directs lytic granule exocytosis and cytotoxicity of mouse killer T cells.</style></title><secondary-title><style face="normal" font="default" size="100%">Eur J Immunol</style></secondary-title><alt-title><style face="normal" font="default" size="100%">Eur. J. Immunol.</style></alt-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Animals</style></keyword><keyword><style  face="normal" font="default" size="100%">Blotting, Western</style></keyword><keyword><style  face="normal" font="default" size="100%">Cytotoxicity, Immunologic</style></keyword><keyword><style  face="normal" font="default" size="100%">Exocytosis</style></keyword><keyword><style  face="normal" font="default" size="100%">Flow Cytometry</style></keyword><keyword><style  face="normal" font="default" size="100%">Mice</style></keyword><keyword><style  face="normal" font="default" size="100%">Mice, Knockout</style></keyword><keyword><style  face="normal" font="default" size="100%">Mice, Transgenic</style></keyword><keyword><style  face="normal" font="default" size="100%">Proteins</style></keyword><keyword><style  face="normal" font="default" size="100%">Reverse Transcriptase Polymerase Chain Reaction</style></keyword><keyword><style  face="normal" font="default" size="100%">Secretory Vesicles</style></keyword><keyword><style  face="normal" font="default" size="100%">T-Lymphocytes, Cytotoxic</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2014</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2014 Oct</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">44</style></volume><pages><style face="normal" font="default" size="100%">3109-18</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Midline 1 (MID1) is a microtubule-associated ubiquitin ligase that regulates protein phosphatase 2A activity. Loss-of-function mutations in MID1 lead to the X-linked Opitz G/BBB syndrome characterized by defective midline development during embryogenesis. Here, we show that MID1 is strongly upregulated in murine cytotoxic lymphocytes (CTLs), and that it controls TCR signaling, centrosome trafficking, and exocytosis of lytic granules. In accordance, we find that the killing capacity of MID1(-/-) CTLs is impaired. Transfection of MID1 into MID1(-/-) CTLs completely rescued lytic granule exocytosis, and vice versa, knockdown of MID1 inhibited exocytosis of lytic granules in WT CTLs, cementing a central role for MID1 in the regulation of granule exocytosis. Thus, MID1 orchestrates multiple events in CTL responses, adding a novel level of regulation to CTL activation and cytotoxicity.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">10</style></issue><custom1><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/25043946?dopt=Abstract</style></custom1></record></records></xml>